ion exchange hplc columns

Ion Exchange Group: Alkanol Quaternary Ammonium Ion: Max. Pre-Packed Columns; Automated Columns; Manual Columns; Chromatography Systems. 7,10 This method relies on the interaction (or 4. Ion-pair chromatography; It is a form of chromatography in which ions in solution can be paired or neutralized and separated as an ion pair on a reversed-phase column. other polar contaminants in food, a novel hybrid ion exchange/HILIC column as well as a simple HPLC system passivation solution has been developed. WebIon Exchange Chromatography (IEX) Hydrophobic Interaction Chromatography (HIC) Chromatography Columns. Ion Exchange Chromatography (IEX) Hydrophobic Interaction Chromatography (HIC) Chromatography Columns. Different packing materials support different separation mechanisms common are materials for normal-phase, reversed-phase, size exclusion, ion exchange, affinity, chiral, or hydrophilic interaction HPLC. The Bio IEX family offers strong cation exchange (SCX), Compare this item Ion-Exchange SAX Columns WebIon exchange chromatography is a very powerful tool for use in protein purification and is frequently used in both analytical and preparative separations. Ion-Exchange Columns. Same charged ions are excluded. Octet SF3 SPR Adsorption chromatography 2. Ion exchange chromatography 3. It is our mission to equip the scientists, quality leaders, and process engineers of the pharmaceutical and biopharmaceutical industries with the most advanced insights and productivity tools for the fine separations that are crucial to the Standard Series C8/C18 HPLC Columns; NH2 HPLC Columns; Amino Acid HPLC Columns; HILIC Amide HPLC Columns; C1 HPLC Columns; Reversed Phase C4 HPLC Columns; C30 HPLC Columns; Ion-Exchange NH2 SPE Cartridges; Ion-Exchange PRS SPE Cartridges; Ion-Exchange PSA SPE Cartridges; Ion-Exchange SAX SPE Cartridges; 1. Preparative Columns. How are HPLC columns made? Ion exchangers provide separations that are complementary to reversed-phase HPLC. Ion Exchange Group: Alkanol Quaternary Ammonium Ion: Max. Multi-Use Batch Chromatography Systems; Single-Use Batch Chromatography Systems Preparative HPLC Chromatography Systems. It relies on pumps to pass a pressurized liquid solvent containing the sample mixture through a column filled with a solid adsorbent material. NH2 is amino. 1 However, their use is limited by dewetting in highly aqueous environments, 2,3 peak tailing for basic molecules 46 and insufficient retention of Separation by HPLC can be done by reverse-phase, ion-exchange or size-exclusion methods, and samples detected by diode array or laser technology. They are the column of choice for monitoring inorganic anions in drinking water and wastewater samples for compliance with U.S. EPA Methods 300.0 (A) and 300.1. NH2 is amino. WebSeparate proteins and their variants using these weak cation-exchange columns with exceptionally high resolution. For high sensitivity, Hamilton PRP-X110 ion chromatography columns are used to separate ions at concentrations from less than 20 ppb to 20 ppm. Separation by HPLC can be done by reverse-phase, ion-exchange or size-exclusion methods, and samples detected by diode array or laser technology. WebStandard Series C8/C18 HPLC Columns; NH2 HPLC Columns; Amino Acid HPLC Columns; HILIC Amide HPLC Columns; C1 HPLC Columns; Reversed Phase C4 HPLC Columns; C30 HPLC Columns; Ion-Exchange NH2 SPE Cartridges; Ion-Exchange PRS SPE Cartridges; Ion-Exchange PSA SPE Cartridges; Ion-Exchange SAX SPE Cartridges; 7,10 This method relies on the interaction (or exchange) of ions in the ODCN is a mixed mode column consisting of C18 and nitrile. Also Read : Best cough syrups name in India 2020. This application describes the separation of three model proteins by a salt gradient with the AZURA Bio Lab system. YMC provides various products, including HPLC columns, ion exchange chromatography columns/packing materials for purification of biopharmaceuticals, and chiral chromatography columns/packing materials, which can separate Polymeric packing materials can be operated in a wide pH range of eluents due to their chemical stability. How are HPLC columns made? SAX is strong anionic exchange (used for separation of carboxylic acid compounds). Ion-Exchange HPLC. SMT SAX Columns: Comparable Columns: The PRP-X110 has similar selectivity to the PRP-X100 but provides lower limits of detection as a result of its lower exchange capacity. Our recommended equivalent HPLC columns will most likely give a similar selectivity. between the two columns will be in the length of the carbon- chain attached to the silica surface. Catalog Number 063000 The mechanism was discussed in Section 5.2, and details about the mobile phases used for ion pairing were given in Section 7.7.Ion pairing is a common separation technique for Inertsil ODS-3 is still GL Sciences most popular phase and continues to be used world widely and reliably for long established methods in pharmaceutical, and contract research labs. Each column is packed with bonded, 5 m, spherical silica particles for optimum efficiency. Chiral LC: Used to separate enantiomers. Chiral LC: Used to separate enantiomers. Hamilton PRP-X100 and PRP-X110 are highly stable, inert materials. Multi-Use Batch Chromatography Systems; Single-Use Batch Chromatography Systems Preparative HPLC Chromatography Systems. Size Exclusion Chromatography (SEC) Used to separate biomolecules and polymers. Pellicular particles surround specific ion exchange resin types. Like a thin homogeneous silicon dioxide shell, polystyrene-divinyl-benzene synthetic resin, or alumina. Also Read : Best cough syrups name in India 2020. Pressure: 3000 psi (207 bar) Solvent Compatibility: pH 0 to 14, 0 to 100% HPLC Solvents: Particle Size: 7.5 m: Stationary Phase: IonPac AS19: Format: Microbore: Packing Material: Polymeric: Pore Size: 2000 Å Length (Metric) 250 mm: Diameter (Metric) 2 mm: Unit Size: Each 1 Unit Size. Stationary phase in these columns remains acidic or basic having negative or positive charge while mobile phase is a polar liquid as the salt solution in water. Figure-2 Absorption Chromatography Figure-3 Ion-exchange chromatography. About YMC The Leading Fine Separations Company. Uwe D. Neue, Wiley-VC; The goal of BIST is simple expand the scope of HPLC applications. Email: sales@separationmethods.com. Octet SF3 SPR Zorbax Strong Anion Exchange packing has a permanently bonded quaternary amine. 5 - 20 Microns; 15 - 40 Microns; HPLC Columns; Scavengers; Solid Phase Extraction (SPE) Application Notes. WebNader Rifai PhD, in Tietz Textbook of Clinical Chemistry and Molecular Diagnostics, 2018. Read user reviews, compare products & request pricing from manufacturers of Ion Exchange Columns | UHPLC and HPLC Our range includes standard and fully customized turbo pumps, ion pumps, diffusion pumps, scroll pumps, rotary vane pumps, and helium leak detectors. Normal phase mode 2.Reverse phase mode Based on principle of separation 1. Ion Exchange Group: Alkanol Quaternary Ammonium Ion: Max. Choose cyano bonded phase for normal phase separations. Portfolio. Element (formerly Crawford Scientific) is the UK's premier supplier of chromatography products, training, consultancy, and technical services.. With our in-house expertise and independent status, we can offer you the products and services which are right for your instrumentation and applications without manufacturer bias. YMC's HPLC (High Performance Liquid Chromatography) columns/packing materials are highly respected products with a worldwide track record. Capillary Columns. Preparative Columns. ODS is octadecyl or C18. Use this gel filtration calibration standard to determine the size and molecular weight of proteins in gel filtration chromatography. PFP is pentafluorphenyl. WebIon Exchange: Used to separate charged compounds. Contains two visible markers, vitamin B12 and myoglobin, to ensure that the column is properly packed and the sample is evenly eluted The mobile phase is an aqueous buffer. Social Hub. All ion exchange chromatography relies on electrostatic interactions between the resin functional groups and proteins of interest; thus, the workflow below is given as a generalized IEX workflow, and particular running conditions for anion exchange chromatography may be adjusted to best suit your protein of interest, the buffer system, and the anion exchange resin chosen. Individual HPLC columns may use any one of a number of different phases or processes to induce this resolution including normally bonded phase, reversed phase, size exclusion, ion Ion exchange HPLC is a very useful but underutilized tool for peptide purification. Ion exchange chromatography 3. Columns are usually made of polished stainless steel, are between 50 and 300 mm long, and have an internal diameter between 2 and 5 mm. Various aspects related to ion-pair chromatography were presented previously. We provide flexible and reliable vacuum solutions for research and development, Bio Ion Exchange HPLC columns are packed with polymeric, nonporous, ion exchange particles. Elution (extracting one material from another) of the The third type of liquid chromatography is IEC. back; DB-WAX Ultra Inert; DB-1ms Low-Bleed; DB-1ms Ultra Inert; HP-1ms Low-Bleed; HP-1ms Ultra Inert; DB-5ms Low-Bleed; Ion Exchange: Used to separate charged compounds. Catalog number: 054993. SPE Columns; SPE Accessories; Life Science/Bio Products. In this type of chromatography, retention is based on the attraction between solute ions and charged sites bound to the stationary phase. Normal Phase column; Reverse phase column; Ion exchange column Ion Exchange Phases; Scavengers; HPLC Guard Cartridges Holders; Selection Guide by Manufacturer; HPLC Column Packing Services; SiliaFlash - Irregular Silica Gels. WebEnjoy strong anion exchange for high-resolution analysis and purification of synthetic oligonucleotides with Thermo Scientific DNAPac PA200 Oligonucleotide HPLC Columns. HPLC is a High Performance liquid Chromatography. Phone: 1 (302) 368-0610. Mobile phases SAX is strong anionic exchange (used for separation of carboxylic acid compounds). Depending on the desired application, numerous HPLC columns are commercially available. The comparable columns may lead to improved & more reliable separation. Contains two visible markers, vitamin B12 and myoglobin, to ensure that the column is properly packed and the sample is evenly eluted WebDiscover vacuum pumps, helium leak detectors, vacuum measurement instruments, and vacuum components from Agilent (formerly Varian Vacuum). Dionex IonPac AS18 columns are high capacity hydroxide-selective anion-exchange columns. In cation exchange chromatography, the stationary bed has an ionically negative (-) charged surface while the sample ions are of positive (+) charge. WebIon Exchange Chromatography (IEX) Hydrophobic Interaction Chromatography (HIC) Chromatography Columns. 6. Within the column, the mixture is resolved into its component parts. Nader Rifai PhD, in Tietz Textbook of Clinical Chemistry and Molecular Diagnostics, 2018. Multi-Use Batch Chromatography Systems; Single-Use Batch Chromatography Systems Preparative HPLC Chromatography Systems. WebHamilton PRP-X100 and PRP-X110 are highly stable, inert materials. An ion exchange column can be either cationic or anionic. WebAll ion exchange chromatography relies on electrostatic interactions between the resin functional groups and proteins of interest; thus, the workflow below is given as a generalized IEX workflow, and particular running conditions for anion exchange chromatography may be adjusted to best suit your protein of interest, the buffer system, and the anion exchange Ion exchange chromatography is the most popular method for the purification of proteins and other charged molecules. Size Exclusion Chromatography (SEC) Used to Types of HPLC columns: There are four types of HPLC columns used in liquid chromatography. PFP is pentafluorphenyl. WebAbout YMC The Leading Fine Separations Company. PRP-X100: Anion Exchange HPLC Column. For high sensitivity, Hamilton PRP-X110 ion chromatography columns are used to separate ions at concentrations from less than 20 ppb to 20 ppm. Pellicular or porous particles are commonly used in HPLC columns. 5. Pellicular particles surround specific ion exchange resin types. SMT SAX Columns: Comparable Columns: Analytical Column (18) Guard Cartridge, 4 Pk. These are widely used in inorganic molecule analysis. It is forbidden to empty the chromatographic column. 088778. Portfolio. WebIon exchange HPLC columns have charged packing. Buffers like phosphate buffers, acetate buffers, etc. Ion Exclusion: Used to separate organic acids, carbohydrates, sugars, starches, and oligosaccharides. WebUse this gel filtration calibration standard to determine the size and molecular weight of proteins in gel filtration chromatography. These are widely used in inorganic molecule analysis. Normal phase mode 2.Reverse phase mode Based on principle of separation 1. Employ Precipitation . Pre-Packed Columns; Automated Columns; Manual Columns; Chromatography Systems. ProPac WCX-10 HPLC Columns. In this type of chromatography, retention is based on the attraction between solute ions and charged sites bound to the stationary phase. ODS is octadecyl or C18. Avoid salt precipitation in the chromatographic column. It can only switch between mutually miscible mobile phases. CN is cyano. HILIC and Ion Exchange (7) Ion Exchange (17) Type. Ion exchange chromatography is the most popular method for the purification of proteins and other charged molecules. WebSerban C. Moldoveanu, Victor David, in Essentials in Modern HPLC Separations, 2013 General Comments. 2. Purification procedures utilizing both ion exchange and reversed-phase modes have greatly increased resolving power compared to procedures that use reversed-phase alone. WebA type of ion exchange chromatography, membrane exchange is a relatively new method of purification designed to overcome limitations of using columns packed with beads. MCI GEL polymeric separation materials for HPLC include ion exchange resins, non-functionalized reversed-phase polymers and hydrophilic porous gels. C18 and C8 are the most common columns used in the pharmaceutical industry. Ion Exchange Chromatography (IEX) Hydrophobic Interaction Chromatography (HIC) Chromatography Columns. Columns. By Particle Size. Cation Exchange. WebDepending on the desired application, numerous HPLC columns are commercially available. WebBridge Ion Separation Technology, or BIST, is a brand new LC separation technique developed by SIELC. Cation exchange columns are the reverse, negatively charged beads that attract positively charged proteins. Stationary phase in these columns remains acidic or basic having negative or positive charge while mobile phase is a polar liquid as the salt solution in water. They are the column of choice for monitoring inorganic anions in drinking water and wastewater samples for compliance with U.S. EPA Methods 300.0 (A) and 300.1. hplc-column. The mobile phase is an aqueous buffer. Ion-Exchange HPLC: It uses to separate the ions and polar molecules based on their affinity to the ion exchanger. The mechanism was discussed in Section 5.2, and details about the mobile phases used for ion pairing were given in Section 7.7.Ion pairing is a common separation technique for certain important IEC is a type of liquid chromatography in which ions are separated by their adsorption onto a support that contains fixed charges on its surface. Normal Phase column; Reverse phase column; Ion High-performance liquid chromatography (HPLC), formerly referred to as high-pressure liquid chromatography, is a technique in analytical chemistry used to separate, identify, and quantify each component in a mixture. Catalog number: 054993. ODCN is a mixed mode column consisting of C18 and nitrile. Adsorption chromatography 2. WebYMC's HPLC (High Performance Liquid Chromatography) columns/packing materials are highly respected products with a worldwide track record. Serban C. Moldoveanu, Victor David, in Essentials in Modern HPLC Separations, 2013 General Comments. Analytical Columns. Partition chromatography 4. Email: sales@separationmethods.com. 3. Octet SF3 SPR Bio Ion Exchange Columns are designed for high resolution, high recovery and highly efficient separations of peptides, oligonucleotides and proteins. WebIon Exchange Group: Alkanol Quaternary Ammonium Ion: Max. We have been innovators and producers of purification science methods and products since 1980. A type of ion exchange chromatography, membrane exchange is a relatively new method of purification designed to overcome limitations of using columns packed with beads. Unit Size. For high sensitivity, Hamilton PRP-X110 ion chromatography columns are used to separate ions at concentrations from less than 20 ppb to 20 ppm. Antibody Purification Columns; Protein Purification Columns; Gas/Air Sampling Equipment. To produce pellicular particles, people often use polymer or glass beads. Mobile phases Capillary Columns. back; DB-WAX Ultra Inert; DB-1ms Low-Bleed; DB-1ms Ultra Inert; HP-1ms Low-Bleed; HP-1ms Ultra Inert; DB-5ms Low-Bleed; Types of HPLC columns: There are four types of HPLC columns used in liquid chromatography. Chromatography consumables on-demand webinar series Discover live and on-demand webinars that enable you to improve productivity, maintain compliance, and learn about the constantly evolving scientific landscape. HPLC is a High Performance liquid Chromatography. Catalog Number 063000 HPLC Columns; HPLC Instruments; HPLC Accessories; Promotions; Gas Chromatography. Pellicular or porous particles are commonly used in HPLC columns. Partition chromatography 4. It reduces the method development and analysis time for the sample. Various aspects related to ion-pair chromatography were presented previously. Ion Exchange Chromatography (IEX) Hydrophobic Interaction Chromatography (HIC) Chromatography Columns. between the two columns will be in the length of the carbon- chain attached to the silica surface. This type of HPLC column separates polar molecules based on their charge. WebAn ion-selective electrode (ISE), also known as a specific ion electrode (SIE), is a transducer (or sensor) that converts the activity [clarification needed] of a specific ion dissolved in a solution into an electrical potential.The voltage is theoretically dependent on the logarithm of the ionic activity, according to the Nernst equation.Ion-selective electrodes are used in 2.12 Accordingly C8 This type of HPLC column separates polar molecules based on their charge. WebAgilent Bond Elut Ion Exchange Silica SPE; Agilent Bond Elut Ion Mixed Mode Silica SPE; Agilent Bond Elut Inorganic SPE; Agilent Specialty SPE; Zirconia Based HPLC Columns; GC Columns. Ion-Exchange HPLC: It uses to separate the ions and polar molecules based on their affinity to the ion exchanger. (6) Application. Enjoy strong anion exchange for high-resolution analysis and purification of synthetic oligonucleotides with Thermo Scientific DNAPac PA200 Oligonucleotide HPLC Columns. It relies on pumps to pass a pressurized liquid solvent containing the sample mixture through a column filled with a solid adsorbent material. WebReversed Phase Columns (C18 Column) Inertsil ODS-3V (Specifically Qualified HPLC Columns for GLP/GMP Compliance Validation) Inertsil ODS-3V offers all of the outstanding chromatographic benefits of Inertsil ODS-3 with the added benefit of a more thoroughly documented, validated QC procedure consistent with the demands of GLP/GMP compliance. The comparable columns may lead to improved & more reliable separation. Like a thin homogeneous silicon dioxide shell, polystyrene-divinyl-benzene synthetic resin, or alumina. Showing 1-30 of 313 results for"ion exchange hplc column" Advanced Search Structure Search Relevance Compare HamiltonHPLC anion exchange column HamiltonHPLC anion exchange WebDionex IonPac AS18 columns are high capacity hydroxide-selective anion-exchange columns. The PRP-X110 has similar selectivity to the PRP-X100 but provides lower limits of detection as a result of its lower exchange capacity. [1] WebHigh-performance liquid chromatography (HPLC), formerly referred to as high-pressure liquid chromatography, is a technique in analytical chemistry used to separate, identify, and quantify each component in a mixture. By Particle Size. Catalog number: 303355. Octet SF3 SPR Multi-Use Batch Chromatography Systems; Single-Use Batch Chromatography Systems Preparative HPLC Chromatography Systems. The newly developed column offers simplicity in LC-MS mobile phase conditions, high lot-to-lot and run-to-run consistency, and versatility in the separation of a wide range of polar analytes. conventional columns to high efficiency columns 88 90 Method development for other HPLC modes 92 HILIC 92 Normal phase chromatography 94 Ion-exchange chromatography 96 Gel permeation chromatography/size exclusion chromatography 97 LC/MS Instrumentation Fundamentals 98 What is LC/MS 98 Ion exchange chromatography is best to use for inorganic anion or cation analysis. Ion exchange chromatography is a very powerful tool for use in protein purification and is frequently used in both analytical and preparative separations. The third type of liquid chromatography is IEC. Different packing materials support different separation mechanisms common are materials for normal-phase, reversed-phase, size exclusion, ion exchange, affinity, chiral, or hydrophilic interaction HPLC. hplc-column. Hamilton PRP-X100 and PRP-X110 are highly stable, inert materials. Reversed Phase Columns (C18 Column) Inertsil ODS-3V (Specifically Qualified HPLC Columns for GLP/GMP Compliance Validation) Inertsil ODS-3V offers all of the outstanding chromatographic benefits of Inertsil ODS-3 with the added benefit of a more thoroughly documented, validated QC procedure consistent with the demands of GLP/GMP compliance. Modern columns have different polarity. Multi-Use Batch Chromatography Systems; Single-Use Batch Chromatography Systems Preparative HPLC Chromatography Systems. Pre-Packed Columns; Automated Columns; Manual Columns; Chromatography Systems. HPLC Columns Theory, Technology and Practice. Columns are usually made of polished stainless steel, are between 50 and 300 mm long, and have an internal diameter between 2 and 5 mm. Ion-Exchange HPLC. ZORBAX Bio-SCX Series II for 2-D separations. back; Agilent J&W GC Columns. Ion-pair chromatography; It is a form of chromatography in which ions in solution can be paired or neutralized and separated as an ion pair on a reversed-phase column. Same charged ions are excluded. Discover vacuum pumps, helium leak detectors, vacuum measurement instruments, and vacuum components from Agilent (formerly Varian Vacuum). Ion-Exchange Columns. [1] Figure-2 Absorption Chromatography Figure-3 Ion-exchange chromatography. Buffers like phosphate buffers, acetate buffers, etc. WebC18 and C8 are the most common columns used in the pharmaceutical industry. The goal of BIST is simple expand the scope of HPLC applications. Ion-Exchange Chromatography. Zorbax strong ion-exchange columns are available as both Strong Anion Exchange (SAX) and Strong Cation Exchange (300SCX) columns. Pre-Packed Columns; Automated Columns; Manual Columns; Chromatography Systems. Pressure: 3000 psi (207 bar) Solvent Compatibility: pH 0 to 14, 0 to 100% HPLC Solvents: Particle Size: 7.5 m: Stationary Phase: IonPac AS19: Format: Microbore: Packing Material: Polymeric: Pore Size: 2000 Å Length (Metric) 250 mm: Diameter (Metric) 2 mm: Unit Size: Each 1 Separate proteins and their variants using these weak cation-exchange columns with exceptionally high resolution. Pre-Packed Columns; Automated Columns; Manual Columns; Chromatography Systems. Pre-Packed Columns; Automated Columns; Manual Columns; Chromatography Systems. Ion exchange chromatography is a popular technique for protein separation and purification. Membrane Chromatographic [36] [37] devices are cheap to mass-produce and disposable unlike other chromatography devices that require maintenance and time to revalidate. Use only filtered and degassed mobile phase. WebIon Exchange Chromatography (IEX) Hydrophobic Interaction Chromatography (HIC) Chromatography Columns. Ion Exclusion: Used to separate organic acids, carbohydrates, sugars, starches, and oligosaccharides. Membrane Chromatographic [36] [37] devices are cheap to mass-produce and disposable unlike other chromatography devices that require maintenance and time to revalidate. Our range includes standard and fully customized turbo pumps, ion pumps, diffusion pumps, scroll pumps, rotary vane pumps, and helium leak detectors. Inertsil ODS-3 is still GL Sciences most popular phase and continues to be used world widely and reliably for long established methods in pharmaceutical, and contract research labs. back; Agilent J&W GC Columns. Discover the unique Thermo Scientific ProPac Elite WCX weak cation exchange (WCX) liquid chromatography column. Phone: 1 (302) 368-0610. Inertsil ODS-4V (Specifically Qualified HPLC Columns for GLP/GMP Compliance Validation) Inertsil ODS-3. Bridge Ion Separation Technology, or BIST, is a brand new LC separation technique developed by SIELC. Ion Exchange: Type: Column Set (3 columns, 1 lot) Technique: Protein/Cation Exchange: Unit Size: Each: Catalog Number. Ion Exchange Phases Scavengers HPLC Guard Cartridges Holders Selection Guide by Manufacturer HPLC Column Packing Services SiliaFlash - Irregular Silica Gels Portfolio By Particle Size 5 - 20 Microns 15 - 40 Microns 20 - 45 Microns 40 - 63 Microns 60 - 120 Microns 60 - 200 Microns 75 - 150 Microns 75 - 250 Microns 105 - 175 Microns 120 - 200 Microns WebOur recommended equivalent HPLC columns will most likely give a similar selectivity. Learn about the common mistakes users make with ion exchange columns and ways to avoid those mistakes. Octet SF3 SPR Ion Exchange Hplc Columns. An ion exchange column can be either cationic or anionic. SCX is strong cationic exchange (used for separation of organic amines). Multi-Use Batch Chromatography Systems; Single-Use Batch Chromatography Systems Preparative HPLC Chromatography Systems. It reduces the method development and analysis time for the sample. ProPac WCX-10 HPLC Columns. Social Hub. This technique is used almost exclusively with ionic or ionizable samples. 088778. Columns. To produce pellicular particles, people often use polymer or glass beads. Ion Exchange: Type: Column Set (3 columns, 1 lot) Technique: Protein/Cation Exchange: Unit Size: Each: Catalog Number. Reversed phase Columns (C18 Column) Inertsil ODS-3 Analytical Columns. SCX is strong cationic exchange (used for separation of organic amines). 5 - 20 Microns; 15 - 40 Microns; HPLC Columns; Scavengers; Solid Phase Extraction (SPE) Application Notes. HPLC Columns Theory, Technology and Practice. IEC is a type of liquid chromatography in which ions are separated by their adsorption onto a support that contains fixed charges on its surface. Mobil phases consist an aqueous buffer system into which the mixture to be resolved. Achieve high-quality LC-based analyses and lab scale purifications of a wide range of peptide and protein biomolecules using Waters Protein-Pak Ion Exchange (IEX) and Hydrophobic Interaction Chromatography (HIC) HPLC and UPLC Columns. Ion-Exchange Chromatography. It relies on pumps to pass a pressurized liquid solvent containing the sample mixture through a column filled with a solid adsorbent material.